Interferon gamma Protein (IFNG) (AA 24-161) (Biotin,His-Avi Tag)

Catalog No. ABIN7274848

This Recombinant Interferon gamma protein is expressed in HEK-293 Cells.

Quick Overview for Interferon gamma Protein (IFNG) (AA 24-161) (Biotin,His-Avi Tag) (ABIN7274848)

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-161

Purification tag / Conjugate: This Interferon gamma protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human IFN gamma/IFNG Protein

Sequence: Gln24-Gly161

Characteristics: Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IFN gamma R1, hFc Tag (Cat. IFN-HM2GR) with the EC50 of 3.7ng/ml determined by ELISA.,Biotinylated Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IFN gamma is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN gamma

Background: Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Molecular Weight: 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy