Interleukin 17a Protein (AA 26-158)

Catalog No. ABIN2667471

Recombinant Interleukin 17a protein expressed in Escherichia coli (E. coli).

Quick Overview for Interleukin 17a Protein (AA 26-158) (ABIN2667471)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Biochemical Assay (BCA)

Product Details

Protein Characteristics: AA 26-158

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL-17A

Background: IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8. IL-17A shares 58 % homology with an open reading frame of the T-lymphotropic Herpes virus Samirii virus (viral IL-17). IL-17A belongs to a family of cytokines, which has five members, designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells, Th17 cells. This lineage selectively produces proinflammatory cytokines including IL-17, IL-17F, IL-21, and IL-22. In the mouse, the differentiation of this new lineage is initiated by TGFbeta-1 and IL-6 or IL-21, which activates Stat3 and induces the expression of the transcription factor retinoic acid-related orphan receptors (RORγt and RORα). IL-23, which also activates Stat3, serves to maintain Th17 cells lineage.