IL1A Protein (AA 115-270)

Catalog No. ABIN2667486

Recombinant IL1A protein expressed in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (AA 115-270) (ABIN2667486)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Intracellular Flow Cytometry (ICFC)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 115-270

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in 0.22 μm filtered protein solution, 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, containing 1 % BSA.

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1alpha

Molecular Weight: The 156 amino acid recombinant protein has a predicted molecular mass of 17,990 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Serine.

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints