IL-22 Protein (AA 34-179)

Catalog No. ABIN2667512

This Recombinant IL-22 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-22 Protein (AA 34-179) (ABIN2667512)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Intracellular Flow Cytometry (ICFC)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 34-179

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA and protease inhibitors.

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL-22

Background: IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79 % amino acid identity with murine IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Molecular Weight: The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.8 kDa. The DTT-reduced protein migrates at approximately 15 kDa and the non-reduced protein migrates at approximately 13 kDa by SDS-PAGE.