KIT Ligand Protein (KITLG) (AA 26-189)

Catalog No. ABIN2667575

This Recombinant KIT Ligand protein is expressed in Escherichia coli (E. coli).

Quick Overview for KIT Ligand Protein (KITLG) (AA 26-189) (ABIN2667575)

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 26-189

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 15 ng/ml, corresponding to a specific activity of 6.7 x 104 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22μm filtered protein solution is in 10 mM NaHPO4 pH 7.2, 0.15M NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: SCF

Background: SCF (KITL) is a hematopoietic growth factor, and it can synergize with a number of other cytokines to stimulate growth of hemopoietic progenitors in vitro and stimulates blood cell production in vivo. SCF is encoded by Sl ('steel'), a gene critical to the development of several distinct cell lineages during embryonic life. KITL was identified as a soluble protein, nevertheless, the predicted amino acid sequence indicates that it is an integral transmembrane protein. KITL is generated by proteolytic cleavage from a transmembrane precursor. Two splice variants have been described for KITL, and proteolytic processing of both transmembrane protein products occurs on the cell surface. The binding of KITL, induces the dimerization of the KIT molecule, followed by a change in the configuration of the intracellular domain and the autophosphorylation of the receptor, opening several docking sites for signal transduction molecules. Dysregulation of SCF-KI signaling and gain-of-function KIT mutations contribute to the genesis of many cancers, like acute myeloid leukemia, gastrointestinal stromal tumors, and mastocytosis.

Molecular Weight: The 165 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 18298.0 Da. The DTT-reduced protein migrates at approximately 18 kDaa by SDS-PAGE. The non-reduced protein migrates at approximately 14 kDaa by SDS-PAGE.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway