Quick Overview for PRMT5 Protein (DYKDDDDK Tag) (ABIN2669658)
Target
See all PRMT5 ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This PRMT5 protein is labelled with DYKDDDDK Tag.
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Characteristics
- Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa.
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Protein Arginine Methyltransferase 5 (PRMT5) (AA 1-637) protein (His tag)
custom-made PRMT5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Arginine Methyltransferase 5 (PRMT5) (AA 1-637) protein (GST tag)PRMT5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Arginine Methyltransferase 5 (PRMT5) (AA 531-637) protein (GST tag)PRMT5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Arginine Methyltransferase 5 (PRMT5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PRMT5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3R8 and H4R3 methyltransferase. HMT Assay Conditions:50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl H3 (1-21) peptide, 20 ng/μl Recombinant PRMT5 protein at 2 hours at room temperature. Activity was detected by fluorography.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
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Alternative Name
- PRMT5
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Background
- PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
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Molecular Weight
- 74.2 kDa
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Pathways
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
Target
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