PRMT5 Protein (AA 1-637) (GST tag)
(1 reference)-
- Target See all PRMT5 Proteins
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-637
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PRMT5 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SKB1 (Human) Recombinant Protein (P01)
- Sequence
- MAAMAVGGAG GSRVSSGRDL NCVPEIADTL GAVAKQGFDF LCMPVFHPRF KREFIQEPAK NRPGPQTRSD LLLSGRDWNT LIVGKLSPWI RPDSKVEKIR RNSEAAMLQE LNFGAYLGLP AFLLPLNQED NTNLARVLTN HIHTGHHSSM FWMRVPLVAP EDLRDDIIEN APTTHTEEYS GEEKTWMWWH NFRTLCDYSK RIAVALEIGA DLPSNHVIDR WLGEPIKAAI LPTSIFLTNK KGFPVLSKMH QRLIFRLLKL EVQFIITGTN HHSEKEFCSY LQYLEYLSQN RPPPNAYELF AKGYEDYLQS PLQPLMDNLE SQTYEVFEKD PIKYSQYQQA IYKCLLDRVP EEEKDTNVQV LMVLGAGRGP LVNASLRAAK QADRRIKLYA VEKNPNAVVT LENWQFEEWG SQVTVVSSDM REWVAPEKAD IIVSELLGSF ADNELSPECL DGAQHFLKDD GVSIPGEYTS FLAPISSSKL YNEVRACREK DRDPEAQFEM PYVVRLHNFH QLSAPQPCFT FSHPNRDPMI DNNRYCTLEF PVEVNTVLHG FAGYFETVLY QDITLSIRPE THSPGMFSWF PILFPIKQPI TVREGQTICV RFWRCSNSKK VWYEWAVTAP VCSAIHNPTG RSYTIGL
- Characteristics
- Human SKB1 full-length ORF ( AAH25979.1, 1 a.a. - 637 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Protein Arginine Methyltransferase 5 (PRMT5) (AA 531-637) protein (GST tag)
PRMT5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Arginine Methyltransferase 5 (PRMT5) (AA 1-637) protein (Strep Tag)Crystallography grade PRMT5 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Protein Arginine Methyltransferase 5 (PRMT5) (AA 2-637) protein (His tag)Crystallography grade PRMT5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Protein Arginine Methyltransferase 5 (PRMT5) (AA 2-637) protein (His tag)Crystallography grade PRMT5 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Protein Arginine Methyltransferase 5 (PRMT5) protein (DYKDDDDK Tag)PRMT5 Origin: Human Host: Baculovirus Recombinant EAA, ScA
Protein Arginine Methyltransferase 5 (PRMT5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PRMT5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Arginine methylation by PRMT5 at a naturally occurring mutation site is critical for liver metabolic regulation by small heterodimer partner." in: Molecular and cellular biology, Vol. 31, Issue 7, pp. 1540-50, (2011) (PubMed).
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: "Arginine methylation by PRMT5 at a naturally occurring mutation site is critical for liver metabolic regulation by small heterodimer partner." in: Molecular and cellular biology, Vol. 31, Issue 7, pp. 1540-50, (2011) (PubMed).
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- Target
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
- Alternative Name
- PRMT5 (PRMT5 Products)
- Synonyms
- HRMT1L5 Protein, IBP72 Protein, JBP1 Protein, SKB1 Protein, SKB1Hs Protein, Jbp1 Protein, Skb1 Protein, si:zc14a17.2 Protein, skb1 Protein, wu:fb95f10 Protein, zgc:110669 Protein, hsl7 Protein, DDBDRAFT_0187422 Protein, DDBDRAFT_0235403 Protein, DDB_0187422 Protein, DDB_0235403 Protein, NV18830 Protein, jbp1 Protein, ibp72 Protein, skb1hs Protein, hrmt1l5 Protein, protein arginine methyltransferase 5 Protein, protein arginine N-methyltransferase 5 Protein, protein arginine methyltransferase 5 L homeolog Protein, putative arginine N-methyltransferase, type II Protein, Skb1 family protein Protein, Protein arginine N-methyltransferase 5 Protein, PRMT5 Protein, Prmt5 Protein, prmt5 Protein, prmt5.L Protein, LOC100115766 Protein, prmt-5 Protein
- Background
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Full Gene Name: protein arginine methyltransferase 5
Synonyms: HRMT1L5,IBP72,JBP1,SKB1,SKB1Hs - Gene ID
- 10419
- Pathways
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
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