SMYD3 Protein (DYKDDDDK Tag)
Quick Overview for SMYD3 Protein (DYKDDDDK Tag) (ABIN2669668)
Target
See all SMYD3 ProteinsProtein Type
Origin
Source
Application
-
-
Purification tag / Conjugate
- This SMYD3 protein is labelled with DYKDDDDK Tag.
-
Characteristics
- Recombinant Smyd3 (accession number NP_001161212.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 50.7 kDa.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System HEK-293 CellsConjugate His tagOrigin HumanPrice starts at $12,595.45Expression System Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin HumanPrice starts at $20,480.57Your project requires further customization? Contact us and discover our custom protein solutions
-
SET and MYND Domain Containing 3 (SMYD3) (AA 1-428) protein (His tag)
custom-made SMYD3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SET and MYND Domain Containing 3 (SMYD3) (AA 1-428) protein (His tag)custom-made SMYD3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SET and MYND Domain Containing 3 (SMYD3) (AA 1-369) protein (GST tag)SMYD3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SET and MYND Domain Containing 3 (SMYD3) protein (Myc-DYKDDDDK Tag)SMYD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
Application Notes
- Recombinant Smyd3 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Specifically mediates the methylation of 'Lys-4' and 'Lys-5' of histone H3 and may methylate non-histone substrates. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl non-histone peptide, 30 ng/μl Recombinant Smyd3 protein at 2 hours at room temperature. Activity was detected by fluorography.
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
-
Storage
- -80 °C
-
Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
-
-
- SMYD3 (SET and MYND Domain Containing 3 (SMYD3))
-
Alternative Name
- SMYD3
-
Background
- SET and MYND domain-containing protein 3 (Smyd3) is a member of the Smyd family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and tri-methylation, but not monomethylation. Smyd3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Smyd3 expression has been shown to be upregulated in certain cancers.
-
Molecular Weight
- 50.7 kDa
Target
-
