PRMT1 Protein (DYKDDDDK Tag)
Quick Overview for PRMT1 Protein (DYKDDDDK Tag) (ABIN2669672)
Target
See all PRMT1 ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This PRMT1 protein is labelled with DYKDDDDK Tag.
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Characteristics
- Recombinant PRMT1 (accession number NP_938074.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 42 kDa.
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Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-371) protein (His tag)
custom-made PRMT1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-353) (Active) protein (His-MBP)PRMT1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, EAA Active
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-353) (Active) protein (His-MBP)PRMT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, EAA Active
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-361) protein (His tag)PRMT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Protein Arginine Methyltransferase 1 (PRMT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PRMT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Arginine Methyltransferase 1 (PRMT1) (AA 1-248), (partial) protein (GST tag)PRMT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Recombinant PRMT1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3R8 and H4R3 methylase. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Octamers, 30 ng/μl Recombinant PRMT1 protein at 2 hours at room temperature. Activity was detected by fluorography.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))
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Alternative Name
- PRMT1
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Background
- PRMT1 (protein arginine N-methyltransferase 1) is a member of the protein arginine N-methyltransferase (PRMT) family capable of monomethylating and asymmetrically dimethylating arginine residue in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. PRMT1 specifically methylates arginine 3 on histone H4 (H4R3), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, PRMT1 represses STAT1 transcriptional activity in the late phase of interferon gamma (IFN-gamma) signaling. PRMT1 may be involved in the regulation of TAF15 transcriptional activity and may act as an activator of estrogen receptor (ER)-mediated transactivation. It may also play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation by modulating p38 MAPK pathways.
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Molecular Weight
- 42 kDa
Target
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