KDM3B Protein (DYKDDDDK Tag)

Catalog No. ABIN2669679

Recombinant KDM3B protein expressed in Baculovirus.

Quick Overview for KDM3B Protein (DYKDDDDK Tag) (ABIN2669679)

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Product Details

Purification tag / Conjugate: This KDM3B protein is labelled with DYKDDDDK Tag.

Characteristics: Recombinant JMJD1B / KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 193.4 kDa.

Application Details

Application Notes: Recombinant JMJD1B / KDM3B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 40 nM Recombinant JMJD1B / KDM3B protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Alternative Name: JMJD1B / KDM3B

Background: Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.

Molecular Weight: 193.4 kDa

Pathways: Warburg Effect