DOT1L Protein (AA 1-416) (GST tag)

Catalog No. ABIN2669718

Recombinant DOT1L protein expressed in Escherichia coli (E. coli).

Quick Overview for DOT1L Protein (AA 1-416) (GST tag) (ABIN2669718)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1-416

Purification tag / Conjugate: This DOT1L protein is labelled with GST tag.

Characteristics: Recombinant DOT1L (1-416), an active truncation that includes amino acids 1-416 (accession number NP_115871.1) ,was expressed in E. coli cells and contains an N-terminal GST tag with an observed molecular weight of 72 kDa.

Application Details

Application Notes: Recombinant DOT1L (1-416), active protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K79 methyltransferase HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl DOT1L (1-416 aa) at 2 hours at room temperature. Activity was detected by fluorography.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background: DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.

Molecular Weight: 72 kDa