AGO2 Protein (DYKDDDDK Tag)

Catalog No. ABIN2669729

This Recombinant AGO2 protein is expressed in Baculovirus.

Quick Overview for AGO2 Protein (DYKDDDDK Tag) (ABIN2669729)

Target: AGO2 (Argonaute 2 (AGO2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Application: RNA Interference (RNAi), Screening Assay (ScA)

Product Details

Purification tag / Conjugate: This AGO2 protein is labelled with DYKDDDDK Tag.

Characteristics: Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 104 kDa.

Application Details

Application Notes: Recombinant AGO2 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling. Specific Activity: RNA endonuclease activity.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: AGO2

Background: AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10, EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleaves the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions.

Molecular Weight: 104 kDa

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization