AGO2 Protein (His tag)

Catalog No. ABIN7194309

Product Details

Target: AGO2 (Argonaute 2 (AGO2))

Protein Type: Recombinant

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This AGO2 protein is labelled with His tag.

Purpose: Recombinant Mouse AGO2/Argonaute 2/EIF2C2 Protein (His Tag)

Sequence: Met 1-Ala 860

Characteristics: A DNA sequence encoding the mouse AGO2 (Q8CJG0) (Met 1-Ala 860) was fused with a polyhistidine tag at the N-terminus.

Purity: > 88 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: AGO2/Argonaute 2/EIF2C2 (AGO2 Products)

Synonyms: EIF2C2 Protein, T19E23.7 Protein, T19E23_7 Protein, argonaute 2 Protein, Q10 Protein, Eif2c2 Protein, Argonaute2 Protein, eif2c1 Protein, eif2c2 Protein, 1110029L17Rik Protein, 2310051F07Rik Protein, AI225898 Protein, AL022874 Protein, AW546247 Protein, ENSMUSG00000072493 Protein, Gerp95 Protein, Gm10365 Protein, mKIAA4215 Protein, AGO2 Protein, AG02 Protein, AGO 2 Protein, Ago-2 Protein, Ago2 Protein, CG13452 Protein, CG7439 Protein, Dm Ago2 Protein, Dmel\\CG7439 Protein, ago Protein, ago-2 Protein, ago2 Protein, dAGO2 Protein, dAgo2 Protein, eIF2C2 Protein, argonaute 2, RISC catalytic component Protein, Argonaute family protein Protein, argonaute 2, RISC catalytic component L homeolog Protein, argonaute RISC catalytic subunit 2 Protein, argonaute RISC catalytic component 2 Protein, Argonaute 2 Protein, AGO2 Protein, Ago2 Protein, ago2.L Protein, ago2 Protein

Background:

Background: Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

Synonym: 1110029L17Rik,2310051F07Rik,AI225898,AL022874,AW546247,Eif2c2,ENSMUSG00000072493,Gerp95,Gm10365,mKIAA4215

Molecular Weight: 99 kDa

UniProt: Q8CJG0

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization