IL-20 Protein
Quick Overview for IL-20 Protein (ABIN2690584)
Target
See all IL-20 (IL20) ProteinsBiological Activity
Origin
Source
Purity
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Specificity
- Optimized DNA sequence encoding Human Interleukin-20 mature chain was expressed in Escherichia Coli.
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Characteristics
- Native human Interleukin-20 generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant IL-20 is a disulfide-linked monomeric protein consisting of 135 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
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Interleukin 20 (IL20) (AA 25-176) protein (His tag)
IL20 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) protein (Fc Tag)IL20 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) protein (His tag)IL20 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) protein (Fc Tag)IL20 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 20 (IL20) (AA 25-176) (Active) protein (His tag)IL20 Origin: Human Host: Escherichia coli (E. coli) > 90 % AcA, CC Active
Interleukin 20 (IL20) (AA 1-176) protein (GST tag)IL20 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Comment
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The ED(50) was determined by by a cell proliferation assay using human IL-20Ralpha and human IL-20Rbeta co-transfected murine BaF3 pro-B cells and was found to be less than 0.6 ng/mL, corresponding to a specific activity of 1.7 × 10 6 IU/mg.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- PBS solution pH 7.5.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.
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Expiry Date
- 12 months
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- IL-20 (IL20) (Interleukin 20 (IL20))
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Alternative Name
- IL-20
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UniProt
- Q9NYY1
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Pathways
- Protein targeting to Nucleus
Target
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