DGKA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for DGKA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2712274)
Target
See all DGKA ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This DGKA protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DAG kinase alpha (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) protein (His tag)
custom-made DGKA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-730) protein (His tag)custom-made DGKA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) protein (GST tag)DGKA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 341-555) protein (His-IF2DI Tag)DGKA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
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Alternative Name
- Dag Kinase alpha
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Background
- The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
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Molecular Weight
- 82.5 kDa
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NCBI Accession
- NP_958852
Target
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