Cullin 5 Protein (CUL5) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712634

This Recombinant Cullin 5 protein is expressed in HEK-293 Cells.

Quick Overview for Cullin 5 Protein (CUL5) (Myc-DYKDDDDK Tag) (ABIN2712634)

Target: Cullin 5 (CUL5)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Cullin 5 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Cullin-5 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Cullin 5 (CUL5)

Alternative Name: Cullin-5

Background: Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 90.8 kDa

NCBI Accession: NP_003469

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development