CXCL12 Protein (Transcript Variant 1)
Quick Overview for CXCL12 Protein (Transcript Variant 1) (ABIN2712665)
Target
See all CXCL12 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human CXCL12 / SDF1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to chemoattract human peripheral blood monocytes using a concentration of 50-100 ng/ml.
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Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) (Active) protein (His tag,GST tag)
CXCL12 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (Active) proteinCXCL12 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His-IF2DI Tag)CXCL12 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-93) protein (His-IF2DI Tag)CXCL12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 1-89) protein (GST tag)CXCL12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-89) (Active) protein (Biotin)CXCL12 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 97 % by SDS PAGE FACS, CMA Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 Origin: Human Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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Alternative Name
- Cxcl12,sdf1
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Molecular Weight
- 7.9 kDa
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NCBI Accession
- NP_068350
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Pathways
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
Target
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