CMBL Protein (Myc-DYKDDDDK Tag)
Quick Overview for CMBL Protein (Myc-DYKDDDDK Tag) (ABIN2712894)
Target
See all CMBL ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CMBL protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CMBL protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (His tag)
CMBL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (GST tag)CMBL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
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Alternative Name
- Cmbl
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Background
- CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010].
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Molecular Weight
- 27.9 kDa
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NCBI Accession
- NP_620164
Target
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