P-Cadherin Protein (CDH3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713023

This Recombinant P-Cadherin protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for P-Cadherin Protein (CDH3) (Myc-DYKDDDDK Tag) (ABIN2713023)

Target: P-Cadherin (CDH3)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This P-Cadherin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Cadherin-3 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Broussard, Kim, Wiley, Marquez, Annis, Pritchard, Disis: "Identification of putative immunologic targets for colon cancer prevention based on conserved gene upregulation from preinvasive to malignant lesions." in: Cancer prevention research (Philadelphia, Pa.), Vol. 6, Issue 7, pp. 666-74, (2013) (PubMed).

Target Details

Target: P-Cadherin (CDH3)

Alternative Name: Cadherin-3

Background: This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS).

Molecular Weight: 88.8 kDa

NCBI Accession: NP_001784