CA5B Protein (Myc-DYKDDDDK Tag)
Quick Overview for CA5B Protein (Myc-DYKDDDDK Tag) (ABIN2713132)
Target
See all CA5B ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CA5B protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Carbonic anhydrase 5B protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 1-317) protein (GST tag)
CA5B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 34-317) protein (His-IF2DI Tag)CA5B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Carbonic Anhydrase VB, Mitochondrial (CA5B) proteinCA5B Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
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Alternative Name
- Carbonic Anhydrase 5b
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Background
- Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
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Molecular Weight
- 32.5 kDa
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NCBI Accession
- NP_009151
Target
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