PYCARD Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713166

Product Details

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PYCARD protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human CARD5 / PYCARD / ASC (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: Card5,pycard,asc (PYCARD Products)

Synonyms: ASC Protein, CARD5 Protein, TMS Protein, TMS-1 Protein, TMS1 Protein, 9130417A21Rik Protein, Asc Protein, TNS1 Protein, masc Protein, asc1 Protein, zAsc Protein, zgc:109874 Protein, PYD and CARD domain containing Protein, PYCARD Protein, Pycard Protein, pycard Protein

Background: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 19.8 kDa

NCBI Accession: NP_660183

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome