PYD and CARD Domain Containing (PYCARD) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2730218
Protein Name
Protein Characteristics
Transcript Variant 3
2
1
1
1
1
1
Origin
Human
4
1
1
1
Source
HEK-293 Cells
2
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This PYCARD protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human PYD and CARD domain containing (PYCARD), transcript variant 3 (transcript variant 3) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 15.5
NCBI Accession NP_660184
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Image no. 1 for PYD and CARD Domain Containing (PYCARD) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag) (ABIN2730218) Validation with Western Blot