CBFA2T2 Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713251

This Recombinant CBFA2T2 protein is produced in HEK-293 Cells.

Quick Overview for CBFA2T2 Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag) (ABIN2713251)

Target: CBFA2T2 (Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 4

Purification tag / Conjugate: This CBFA2T2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human CBFA2T2 (transcript variant 4) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CBFA2T2 (Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2))

Alternative Name: Cbfa2t2

Background: In acute myeloid leukemia, especially in the M2 subtype, the t(821)(q22q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5&apos-region of the RUNX1 (AML1) gene fused to the 3&apos-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.

Molecular Weight: 63.7 kDa

NCBI Accession: NP_001034798