DARC Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713526

Recombinant DARC protein expressed in HEK-293 Cells.

Quick Overview for DARC Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2713526)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Standard (STD), Antibody Production (AbP)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This DARC protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human CD234 / DARC (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Alternative Name: Cd234,darc

Background: Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and also for the malaria parasites P.vivax and P.knowlesi. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 35.4 kDa

NCBI Accession: NP_002027