CDK2AP1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for CDK2AP1 Protein (Myc-DYKDDDDK Tag) (ABIN2713862)
Target
See all CDK2AP1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CDK2AP1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CDK2AP1 / DOC1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Cyclin-Dependent Kinase 2 Associated Protein 1 (CDK2AP1) (AA 1-115) protein (His tag)
custom-made CDK2AP1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin-Dependent Kinase 2 Associated Protein 1 (CDK2AP1) (AA 1-115) protein (GST tag)CDK2AP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 2 Associated Protein 1 (CDK2AP1) (AA 1-115), (full length) protein (GST tag)CDK2AP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CDK2AP1 (Cyclin-Dependent Kinase 2 Associated Protein 1 (CDK2AP1))
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Alternative Name
- Cdk2ap1,doc1
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Background
- The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Molecular Weight
- 12.2 kDa
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NCBI Accession
- NP_004633
Target
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