CENPM Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for CENPM Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2713909)
Target
See all CENPM ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This CENPM protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CENP-M (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Centromere Protein M (CENPM) (AA 1-180) protein (His tag)
custom-made CENPM Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Centromere Protein M (CENPM) (AA 1-180) protein (His tag)custom-made CENPM Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Centromere Protein M (CENPM) (AA 1-180) protein (GST tag)CENPM Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Centromere Protein M (CENPM) (AA 1-180) protein (His-IF2DI Tag)CENPM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CENPM (Centromere Protein M (CENPM))
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Alternative Name
- Cenp-M
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Background
- The protein encoded by this gene is an inner protein of the kinetochore, the multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It belongs to the constitutive centromere-associated network protein group, whose members interact with outer kinetochore proteins and help to maintain centromere identity at each cell division cycle. The protein is structurally related to GTPases but cannot bind guanosine triphosphate. A point mutation that affects interaction with another constitutive centromere-associated network protein, CENP-I, impairs kinetochore assembly and chromosome alignment, suggesting that it is required for kinetochore formation. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 19.6 kDa
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NCBI Accession
- NP_076958
Target
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