CHKB Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713989

This Recombinant CHKB protein is produced in HEK-293 Cells.

Quick Overview for CHKB Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2713989)

Target: CHKB (Choline Kinase beta (CHKB))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This CHKB protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Choline kinase beta (CHKB), transcript variant 2 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CHKB (Choline Kinase beta (CHKB))

Background: Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus.

Molecular Weight: 13.3 kDa

NCBI Accession: NP_689466