14-3-3 gamma Protein (YWHAG1) (Myc-DYKDDDDK Tag)
Quick Overview for 14-3-3 gamma Protein (YWHAG1) (Myc-DYKDDDDK Tag) (ABIN2714214)
Target
See all 14-3-3 gamma (YWHAG1) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This 14-3-3 gamma protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human 14-3-3 protein gamma protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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14-3-3 gamma (YWHAG1) (AA 1-247) protein (His tag)
custom-made YWHAG1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 gamma (YWHAG1) (AA 1-247) protein (His tag)custom-made YWHAG1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 gamma (YWHAG1) (AA 2-247) protein (His tag)YWHAG1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
14-3-3 gamma (YWHAG1) (AA 1-247) proteinYWHAG1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
14-3-3 gamma (YWHAG1) (AA 1-247) protein (GST tag)YWHAG1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 14-3-3 gamma (YWHAG1)
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Alternative Name
- 14-3-3 Protein gamma
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Background
- This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
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Molecular Weight
- 28.1 kDa
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NCBI Accession
- NP_036611
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Pathways
- Myometrial Relaxation and Contraction, M Phase
Target
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