14-3-3 theta Protein (YWHAQ) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714217

This Recombinant 14-3-3 theta protein is produced in HEK-293 Cells.

Quick Overview for 14-3-3 theta Protein (YWHAQ) (Myc-DYKDDDDK Tag) (ABIN2714217)

Target: 14-3-3 theta (YWHAQ)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This 14-3-3 theta protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human 14-3-3 protein theta protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 Protein theta

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Molecular Weight: 27.6 kDa

NCBI Accession: NP_006817

Pathways: Apoptosis, Myometrial Relaxation and Contraction