Aconitase 1 Protein (ACO1) (Myc-DYKDDDDK Tag)
Quick Overview for Aconitase 1 Protein (ACO1) (Myc-DYKDDDDK Tag) (ABIN2714323)
Target
See all Aconitase 1 (ACO1) ProteinsProtein Type
Origin
Source
Application
Purity
-
-
Purification tag / Conjugate
- This Aconitase 1 protein is labelled with Myc-DYKDDDDK Tag.
-
Characteristics
-
- Recombinant human ACO1 / IREB1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System HEK-293 CellsConjugate His tagOrigin HumanPrice starts at $12,595.45Expression System Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin HumanPrice starts at $20,480.57Your project requires further customization? Contact us and discover our custom protein solutions
-
Aconitase 1 (ACO1) (AA 1-889) protein (His tag)
custom-made ACO1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aconitase 1 (ACO1) (AA 1-889) protein (His tag)custom-made ACO1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aconitase 1 (ACO1) (AA 780-889) protein (GST tag)ACO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Aconitase 1 (ACO1) (AA 1-889) protein (GST tag)ACO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Aconitase 1 (ACO1) (AA 1-889) protein (His tag)ACO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
-
-
Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
-
The tag is located at the C-terminal.
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 50 μg/mL
-
Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
-
Storage
- -80 °C
-
Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
-
- Aconitase 1 (ACO1)
-
Alternative Name
- Aco1,ireb1
-
Background
- The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
-
Molecular Weight
- 98.2 kDa
-
NCBI Accession
- NP_002188
-
Pathways
- Transition Metal Ion Homeostasis
Target
-
