Smooth Muscle Actin Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for Smooth Muscle Actin Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2714356)
Target
See all Smooth Muscle Actin (ACTA2) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This Smooth Muscle Actin protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human ACTA2 / aortic smooth muscle Actin (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 3-100) protein (His-IF2DI Tag)
ACTA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-377) protein (GST tag)ACTA2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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Alternative Name
- Acta2,aortic Smooth Muscle Actin
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Background
- The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
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Molecular Weight
- 41.8 kDa
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NCBI Accession
- NP_001135417
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Pathways
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
Target
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