BAF53A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714371

This Recombinant BAF53A protein is expressed in HEK-293 Cells.

Quick Overview for BAF53A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2714371)

Target: BAF53A (Actin-Like 6A (BAF53A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This BAF53A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human ACTL6A / BAF53 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: BAF53A (Actin-Like 6A (BAF53A))

Alternative Name: Actl6a,baf53

Background: This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Molecular Weight: 47.3 kDa

NCBI Accession: NP_004292

Pathways: Chromatin Binding, Photoperiodism