ARF4 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714428

This Recombinant ARF4 protein is expressed in HEK-293 Cells.

Quick Overview for ARF4 Protein (Myc-DYKDDDDK Tag) (ABIN2714428)

Target: ARF4 (ADP-Ribosylation Factor 4 (ARF4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This ARF4 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human ARF4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ARF4 (ADP-Ribosylation Factor 4 (ARF4))

Background: This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene.

Molecular Weight: 20.3 kDa

NCBI Accession: NP_001651

Pathways: EGFR Signaling Pathway, Transition Metal Ion Homeostasis