PRAS40 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all PRAS40 (AKT1S1) Proteins
- PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRAS40 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human AKT1S1 / PRAS40 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product AKT1S1 Protein
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AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 1-257) protein (His tag)
custom-made AKT1S1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 1-256) protein (His tag)custom-made AKT1S1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (AA 1-256) protein (His tag)AKT1S1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)AKT1S1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)AKT1S1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
- Alternative Name
- Akt1s1,pras40 (AKT1S1 Products)
- Background
- AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008].
- Molecular Weight
- 27.2 kDa
- NCBI Accession
- NP_115751
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
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