Quick Overview for PLAP Protein (Myc-DYKDDDDK Tag) (ABIN2714573)
Target
See all PLAP (ALPP) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PLAP protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Alkaline phosphatase / PLAP / ALPP protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Placental Alkaline Phosphatase (ALPP) (AA 23-505) protein (His tag)
ALPP Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Placental Alkaline Phosphatase (ALPP) (AA 1-535) protein (GST tag)ALPP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Placental Alkaline Phosphatase (ALPP) (AA 21-504) protein (His tag)ALPP Origin: Cynomolgus Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Placental Alkaline Phosphatase (ALPP) (AA 23-506) (Active) protein (His tag)ALPP Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % SDS, EAA Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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Alternative Name
- Alkaline Phosphatase,plap,alpp
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Background
- The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
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Molecular Weight
- 55.5 kDa
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NCBI Accession
- NP_001623
Target
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