Quick Overview for TUBA1A Protein (Myc-DYKDDDDK Tag) (ABIN2714588)
Target
See all TUBA1A (Tuba1a) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This TUBA1A protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human alpha Tubulin / TUBA1A / TUBA3 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Tubulin, alpha 1a (Tuba1a) (AA 1-451) protein (His tag)
custom-made Tuba1a Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tubulin, alpha 1a (Tuba1a) (AA 1-451) protein (His tag)custom-made Tuba1a Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tubulin, alpha 1a (Tuba1a) (AA 352-451) protein (GST tag)Tuba1a Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Tubulin, alpha 1a (Tuba1a) (AA 214-413) protein (His-IF2DI Tag)Tuba1a Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Tubulin, alpha 1a (Tuba1a) (AA 1-451) protein (GST tag)Tuba1a Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))
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Alternative Name
- alpha Tubulin,tuba1a,tuba3
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Background
- Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Molecular Weight
- 50 kDa
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NCBI Accession
- NP_006000
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Pathways
- Microtubule Dynamics, M Phase
Target
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