APOC3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714826

This Recombinant APOC3 protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for APOC3 Protein (Myc-DYKDDDDK Tag) (ABIN2714826)

Target: APOC3 (Apolipoprotein C-III (APOC3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This APOC3 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Apolipoprotein C III / ApoC3 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Pan, Zhou, Mahsut, Rohm, Berejnaia, Price, Chen, Castro-Perez, Lassman, McLaren, Conway, Jensen, Thomas, Reyes-Soffer, Ginsberg, Gutstein, Cleary, Previs, Roddy: "Static and turnover kinetic measurement of protein biomarkers involved in triglyceride metabolism including apoB48 and apoA5 by LC/MS/MS." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1179-87, (2016) (PubMed).

Target Details

Target: APOC3 (Apolipoprotein C-III (APOC3))

Alternative Name: Apolipoprotein C III (Apo Ciii)

Background: Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia.

Molecular Weight: 8.7 kDa

NCBI Accession: NP_000031

Pathways: Carbohydrate Homeostasis, Lipid Metabolism