ARFGAP3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714863

This Recombinant ARFGAP3 protein is expressed in HEK-293 Cells.

Quick Overview for ARFGAP3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2714863)

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This ARFGAP3 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human ARFGAP3 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Alternative Name: Arfgap3

Background: The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.

Molecular Weight: 56.7 kDa

NCBI Accession: NP_055385