ASS1 Protein (Transcript Variant 1) (His tag)
Quick Overview for ASS1 Protein (Transcript Variant 1) (His tag) (ABIN2715008)
Target
See all ASS1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This ASS1 protein is labelled with His tag.
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Characteristics
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- Recombinant human ASS1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
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Argininosuccinate Synthase 1 (ASS1) (AA 1-412) protein (His tag)
custom-made ASS1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Argininosuccinate Synthase 1 (ASS1) (AA 1-412) protein (His tag)custom-made ASS1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Argininosuccinate Synthase 1 (ASS1) (AA 1-412) protein (GST tag)ASS1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Argininosuccinate Synthase 1 (ASS1) (AA 1-412) protein (His-IF2DI Tag)ASS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 20 mM Phosphate buffer, 150 mM NaCl, 50 mM Imidazole, 1 mM DTT, 40 % Glycerol, pH 7.5. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ASS1 (Argininosuccinate Synthase 1 (ASS1))
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Alternative Name
- Ass1
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Background
- The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
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Molecular Weight
- 42.8 kDa
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NCBI Accession
- NP_000041
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Pathways
- Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
Target
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