ATP6V0A4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715050

Recombinant ATP6V0A4 protein expressed in HEK-293 Cells.

Quick Overview for ATP6V0A4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2715050)

Target: ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This ATP6V0A4 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human ATP6V0A4 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))

Alternative Name: Atp6v0a4

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.

Molecular Weight: 96.2 kDa

NCBI Accession: NP_065683

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport