BCAR3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715176

Recombinant BCAR3 protein expressed in HEK-293 Cells.

Quick Overview for BCAR3 Protein (Myc-DYKDDDDK Tag) (ABIN2715176)

Target: BCAR3 (Breast Cancer Anti-Estrogen Resistance 3 (BCAR3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This BCAR3 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human BCAR3 / SH2D3B protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: BCAR3 (Breast Cancer Anti-Estrogen Resistance 3 (BCAR3))

Alternative Name: Bcar3,sh2d3b

Background: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 92.4 kDa

NCBI Accession: NP_003558