DGKB Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for DGKB Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2715545)
Target
See all DGKB ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This DGKB protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DAG kinase beta (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Diacylglycerol Kinase, beta 90kDa (DGKB) (AA 1-804) protein (His tag)
custom-made DGKB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diacylglycerol Kinase, beta 90kDa (DGKB) (AA 1-802) protein (His tag)custom-made DGKB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DGKB (Diacylglycerol Kinase, beta 90kDa (DGKB))
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Alternative Name
- Dag Kinase beta
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Background
- Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene.
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Molecular Weight
- 90.4 kDa
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NCBI Accession
- NP_004071
Target
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