YWHAE Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for YWHAE Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2715553)
Target
See all YWHAE ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This YWHAE protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human 14-3-3 protein epsilon (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)
custom-made YWHAE Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)custom-made YWHAE Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 epsilon (YWHAE) (AA 1-255) protein (GST tag)YWHAE Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)YWHAE Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
14-3-3 epsilon (YWHAE) protein (GST tag)YWHAE Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by SDS-PAGE
14-3-3 epsilon (YWHAE) (AA 1-255) proteinYWHAE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- YWHAE (14-3-3 epsilon (YWHAE))
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Alternative Name
- 14-3-3 Protein epsilon
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Background
- This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
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Molecular Weight
- 29 kDa
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NCBI Accession
- NP_006752
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Pathways
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
Target
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