DGKI Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719199

Recombinant DGKI protein expressed in HEK-293 Cells.

Quick Overview for DGKI Protein (Myc-DYKDDDDK Tag) (ABIN2719199)

Target: DGKI (Diacylglycerol Kinase, iota (DGKI))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DGKI protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DAG kinase iota protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DGKI (Diacylglycerol Kinase, iota (DGKI))

Alternative Name: Dag Kinase iota

Background: This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.

Molecular Weight: 116.8 kDa

NCBI Accession: NP_004708