DARS Protein (Myc-DYKDDDDK Tag)
Quick Overview for DARS Protein (Myc-DYKDDDDK Tag) (ABIN2719227)
Target
See all DARS ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This DARS protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DARS1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Aspartyl-tRNA Synthetase (DARS) (AA 1-501) protein (His tag)
custom-made DARS Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aspartyl-tRNA Synthetase (DARS) (AA 1-501) protein (His tag)custom-made DARS Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aspartyl-tRNA Synthetase (DARS) (AA 1-501) protein (GST tag)DARS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Aspartyl-tRNA Synthetase (DARS) (AA 1-501) protein (His tag)DARS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DARS (Aspartyl-tRNA Synthetase (DARS))
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Alternative Name
- Dars1
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Background
- This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 57 kDa
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NCBI Accession
- NP_001340
Target
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