DDOST Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719271

Recombinant DDOST protein expressed in HEK-293 Cells.

Quick Overview for DDOST Protein (Myc-DYKDDDDK Tag) (ABIN2719271)

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DDOST protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DDOST protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: Ddost

Background: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 46.1 kDa

NCBI Accession: NP_005207

Pathways: Cell RedoxHomeostasis