DNMT1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719616

Recombinant DNMT1 protein expressed in HEK-293 Cells.

Quick Overview for DNMT1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2719616)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This DNMT1 protein is labelled with Myc-DYKDDDDK Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human DNMT1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Biological Activity Comment: DNMT1 activity verified in a biochemical assay:,DNMT1 activity verified in a biochemical assay:,DNMT1 activity verified in a biochemical assay:

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: Dnmt1

Background: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Molecular Weight: 184.6 kDa

NCBI Accession: NP_001124295

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection