DPM2 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719701

This Recombinant DPM2 protein is produced in HEK-293 Cells.

Quick Overview for DPM2 Protein (Myc-DYKDDDDK Tag) (ABIN2719701)

Target: DPM2 (Dolichyl-Phosphate Mannosyltransferase Polypeptide 2, Regulatory Subunit (DPM2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DPM2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DPM2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DPM2 (Dolichyl-Phosphate Mannosyltransferase Polypeptide 2, Regulatory Subunit (DPM2))

Alternative Name: Dpm2

Background: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1.

Molecular Weight: 9.1 kDa

NCBI Accession: NP_003854

Pathways: Inositol Metabolic Process