DYRK1A Protein (DYKDDDDK Tag)

Catalog No. ABIN2719885

This Recombinant DYRK1A protein is expressed in Insect cells (Sf9).

Quick Overview for DYRK1A Protein (DYKDDDDK Tag) (ABIN2719885)

Target: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DYRK1A protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human DYRK1A (full length, C-term DDK tag, transcript variant 1) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Alternative Name: Dyrk1a

Background: This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5&apos UTR or in the 3&apos coding region. These variants encode at least five different isoforms.

Molecular Weight: 85.6 kDa

NCBI Accession: NP_001387

Pathways: Mitotic G1-G1/S Phases