Dystrophin Protein (DMD) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719890

This Recombinant Dystrophin protein is produced in HEK-293 Cells.

Quick Overview for Dystrophin Protein (DMD) (Myc-DYKDDDDK Tag) (ABIN2719890)

Target: Dystrophin (DMD)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Dystrophin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Dystrophin / DMD (transcript variant Dp140b) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Dystrophin (DMD)

Alternative Name: Dystrophin,dmd

Background: This gene spans a genomic range of greater than 2 Mb and encodes a large protein containing an N-terminal actin-binding domain and multiple spectrin repeats. The encoded protein forms a component of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. Deletions, duplications, and point mutations at this gene locus may cause Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), or cardiomyopathy. Alternative promoter usage and alternative splicing result in numerous distinct transcript variants and protein isoforms for this gene.

Molecular Weight: 143 kDa

NCBI Accession: NP_004012

Pathways: Skeletal Muscle Fiber Development